Recombinant purple acid phosphatase isoform 3 from sweet potato is an enzyme with a diiron metal center
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چکیده
منابع مشابه
A purple acid phosphatase from sweet potato contains an antiferromagnetically coupled binuclear Fe-Mn center.
A purple acid phosphatase from sweet potato is the first reported example of a protein containing an enzymatically active binuclear Fe-Mn center. Multifield saturation magnetization data over a temperature range of 2 to 200 K indicates that this center is strongly antiferromagnetically coupled. Metal ion analysis shows an excess of iron over manganese. Low temperature EPR spectra reveal only re...
متن کاملPhosphate forms an unusual tripodal complex with the Fe-Mn center of sweet potato purple acid phosphatase.
Purple acid phosphatases (PAPs) are a family of binuclear metalloenzymes that catalyze the hydrolysis of phosphoric acid esters and anhydrides. A PAP in sweet potato has a unique, strongly antiferromagnetically coupled Fe(III)-Mn(II) center and is distinguished from other PAPs by its increased catalytic efficiency for a range of activated and unactivated phosphate esters, its strict requirement...
متن کاملBinuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean.
Purple acid phosphatases comprise a family of binuclear metal-containing acid hydrolases, representatives of which have been found in animals, plants, and fungi. The goal of this study was to characterize purple acid phosphatases from sweet potato tubers and soybean seeds and to establish their relationship with the only well-characterized plant purple acid phosphatase, the FeIII-ZnII-containin...
متن کاملThe glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase.
We recently presented clear evidence that the major low-phosphate-inducible phosphatase of the duckweed Spirodela oligorrhiza is a glycosylphosphatidylinositol (GPI)-anchored protein, and, to our knowledge, is the first described from higher plants (N. Morita, H. Nakazato, H. Okuyama, Y. Kim, G.A. Thompson, Jr. [1996] Biochim Biophys Acta 1290: 53-62). In this report the purified 57-kD phosphat...
متن کاملFunctional Assessment of an Overexpressed Arabidopsis Purple Acid Phosphatase Gene (Atpap26) in Tobacco Plants
Background: Overexpression of known genes encoding key phosphate (Pi)-metabolizing enzymes, such as acid phosphatases (APases), is presumed to help plants with Pi availability and absorption as they are mostly exposed to suboptimal environmental conditions for this vital element.Objectives: In this study, the overexpression effect of AtPAP26, one of the m...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2006
ISSN: 1742-464X,1742-4658
DOI: 10.1111/j.1742-4658.2006.05179.x